FastQCFastQC Report
Thu 26 May 2016
ERR1356910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468548
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGC200.007366998651.731035
ATACCTA405.0114475E-543.1045921
CGATAAT1850.033.57317767
TGGTCGT1900.032.69665551
ATAGCAC751.6712183E-632.1881943
CTCGATA1950.031.8548865
GCTCGAT1950.031.8548864
GGCGCTA652.3244265E-531.83447813
GCGCTAG652.3244265E-531.83447814
GTCTACC2100.031.23284556
CTGGTCG2000.031.06182550
CCGGTCA450.00459928830.6947389
CGCTCCG450.004613735730.67506865
CGTCATT2100.029.55743226
GTCGTCT2150.028.8700553
TCGTCTA2200.028.23802254
CGTCGTA500.007695870527.62526510
CTGTGCG2400.027.3375029
GCTCGTC2150.027.2661639
TCGATAA2300.027.00739966
Produced by FastQC (version 0.11.5)