FastQCFastQC Report
Thu 26 May 2016
ERR1356909_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356909_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307537
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT4450.032.5625267
TCGATAA4850.031.29966466
TCGTCTA5150.029.48315254
TGGTCGT5350.028.38097851
GTCGTCT5500.028.23438353
CTCGATA5550.027.3519665
GCTCGAT5600.027.10463164
CGTTAAC1555.2750693E-1126.7348231
CGTCTAC5700.026.63828555
GTCTACC6150.024.68914456
CTGGTCG6150.024.68536450
GTACCGT851.43986E-424.3618726
CGTACGA2700.024.27274360
ATAGCGC2001.8189894E-1224.14591622
CGTCATT6400.023.71383326
CGACGAG4400.023.5187524
ATTAGGT6800.022.32146319
TGCGACG4500.022.22957222
GTACTAG4700.022.0420111
AGGTCGT7000.021.68204922
Produced by FastQC (version 0.11.5)