FastQCFastQC Report
Thu 26 May 2016
ERR1356909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356909_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307537
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG5000.026.949821
TGCGACG4850.025.59906422
CAATGCG5500.024.45486519
ATCGTCA5650.024.44499810
TATGCCG14050.024.05542843
AGTCCTA5400.023.63041329
CTCGATA5850.023.59211365
TCGATAA5600.023.41306566
CGATAAT5700.022.99966867
TCGTATG14700.022.99175540
TCGTCTA5950.022.61744554
CTCGTAT15150.022.3088339
TCTCGTA15100.021.92590938
CGTATGC15450.021.87564741
GCTCGAT6650.021.7916664
CGACGAG5750.021.59225524
TGGTCGT6300.021.36092251
GTCTACC6600.020.9151956
CGTCATT6700.020.5896326
ATGCGAC7050.020.5458221
Produced by FastQC (version 0.11.5)