FastQCFastQC Report
Thu 26 May 2016
ERR1356906_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356906_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324529
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG8390.25852851363052304No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG5840.1799531012636775No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA5440.16762754638260371No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC5010.15437757488544937No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA4930.1519124639092346No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA4900.15098804729315407No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCA4010.12356368768276488No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTG3990.12294740993871117No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG3970.12233113219465748No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGAT3900.12017416009046956No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT3870.11924974347438905No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG3840.1183253268583085No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT3650.11247068828979843No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGAC3390.10445907761710048No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGC3370.10384279987304679No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTGCTAGCCCGTC3310.10199396664088572No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA3260.10045327228075149No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA200.00735240751.7533576
TGCGTTA200.007356893351.74537715
CTCTGGT352.2704395E-549.2813114
TTCAGCG306.2883407E-446.0242854
GTGGAGC306.3074974E-445.99588419
TGTTGCG306.312294E-445.98879238
TCTGGTG404.996681E-543.12114715
GTCTGTA350.001344938239.437211
AGCGCTA350.001344938239.4372157
ACGCCAA350.0013469839.4250518
TTACTAT350.0013469839.4250517
CTGGTTT350.0013469839.4250515
ACGAAGG350.0013469839.4250521
GTTGCGT350.001349024239.4128939
GAGTCCT1350.038.32990628
CAATGCG1350.038.32990619
TGGCCCT400.002594734834.49691864
CTGGTGG400.002594734834.49691816
ATAGCGT501.8594571E-434.4969147
TTGACAT501.8594571E-434.49691419
Produced by FastQC (version 0.11.5)