FastQCFastQC Report
Thu 26 May 2016
ERR1356904_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356904_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505509
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA61030.40537784895341045No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC34590.2297561821284363No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG34090.22643504622024843No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG29070.1930908417020423No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG28260.18771060153077798No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAG25930.1722341081986225No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG24010.15948094631118112No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23510.15615981040299326No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCG21530.14300811220656934No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA18280.12142072880334824No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC16400.10893325778856189No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG15440.10255667684484118No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTCG1550.040.0622640
TCGCCGT1550.037.83783344
CTCGGTG1750.037.44507236
ATCGTCA4700.036.69883710
CCGTATC1800.036.41219747
TCTCGGT1900.036.3040935
CAATGCG4950.036.2415919
GATCTCG2300.035.9991733
TGGTCGC1950.033.6134941
GGTCGCC2000.032.7742442
GGTCCTA958.807547E-932.6812828
GTCATCC5550.031.69880913
TAAGCGT553.2509645E-431.3743846
ACGTTAG553.2579442E-431.36291142
GAGTCCT5300.031.2445628
AGATCTC2550.031.11692632
GTATTAT1800.030.68841
ATTGTGG450.00461539830.6771746
GTCGCCG2050.030.29198343
TGGTCGT6900.029.505251