FastQCFastQC Report
Thu 26 May 2016
ERR1356904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505509
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA72120.4790406433970172RNA PCR Primer, Index 17 (96% over 28bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA58770.3903663146484013No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC35220.23394081337275302No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG32730.21740155654997748No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG30480.2024564449631321No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG26660.1770829666245768No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26090.17329687168924263No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG24270.16120793698343883No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAG24000.1594145235930174No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCG22700.15077957023172894No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA19990.13277901360935074RNA PCR Primer, Index 17 (96% over 28bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA17750.1179003247406691No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC15270.10142749063605731No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG150.002359514468.9717728
ATGGGCG404.976652E-543.1632612
CAATGCG3950.041.906919
TCGCTGG856.548362E-1140.6242512
AACTGAC856.730261E-1140.5716322
GACGAAG1050.039.4124426
ATCGTCA4400.039.23933410
GAGTCCT4200.038.59134728
CGACGAG4600.035.9852724
TATTCGC1003.783498E-1034.5306139
GGCACGG400.002595878734.50192668
TGTGTAT905.2041287E-934.491615
GGTTTGC400.002601805834.4858818
TGGTCGT5550.034.1967851
GTACGCA1350.033.2141463
ACTGACG1056.4574124E-1032.843723
CGAAGCT1056.4574124E-1032.843728
GTCATCC5150.032.81181313
GTCAGCG6100.032.7898643
ATTCGCT1101.0495569E-931.39146610
Produced by FastQC (version 0.11.5)