FastQCFastQC Report
Thu 26 May 2016
ERR1356903_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356903_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487691
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT500.00773736527.59873610
CTCGATA4550.025.02672465
GCTCGAT4700.024.22392364
TGCGACG4150.024.10408822
TCGTCTA5100.023.6833554
TGGTCGT5100.023.6833551
CAATGCG4200.022.99894519
GTACTAG6650.022.8436891
GTCGTCT5450.022.79561453
CGATAAT5100.022.32776367
ATCGTCA4650.022.25704410
GTATCAA34000.022.0351521
TTTTACG800.00290059921.56151417
TCGATAA5150.021.4409666
GCTCGTC5800.021.40489439
CGTCATT5650.021.3663926
GCGCTGG5850.021.22836947
GTCTACC6200.021.15059356
CGACGAG4700.020.54948424
CAACGCA35350.020.2162935
Produced by FastQC (version 0.11.5)