FastQCFastQC Report
Thu 26 May 2016
ERR1356903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487691
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA4700.027.89141765
CGACGAG3700.027.03295324
TGCCGCG1800.026.85830312
GCTCGAT4850.026.31750964
CAATGCG4050.024.69760319
TCGATAA5200.024.54613966
TATTGCG851.4463291E-424.3461153
CGATAAT5500.023.20491867
TGCGACG4550.022.7408722
TCGTCTA5800.022.60394554
GTCGTCT5800.022.00244553
CGCGTCT2057.4578566E-1121.87264615
TGGTCGT6000.021.8504851
CTAACGT953.0678435E-421.7877564
GCCGCGT2251.0913936E-1121.46280913
GCTCGTC6350.021.18303139
GTTTCGC2700.020.4387132
GTATAAT3950.020.1201631
ATCGTCA5150.020.1157910
GTACTAG6050.019.990031
Produced by FastQC (version 0.11.5)