FastQCFastQC Report
Thu 26 May 2016
ERR1356902_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356902_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132436
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG5050.029.37437819
TGCGACG5550.026.10400622
ATCGTCA5450.025.95248810
GTACTAG7800.025.2300741
CGACGAG5800.024.97883424
AATGCGA6550.024.22747620
CTCGATA5650.023.20697265
GCTCGAT5750.022.79748764
TCGATAA5800.022.60679466
CGATAAT6000.021.85323167
CGCACTA6100.021.48388958
GCGATAT2900.021.41193815
TGGTCGT5900.021.05890551
ATGCGAC8000.020.69674921
ACGAGTC7000.020.69626226
TCGTCTA6200.020.5965954
GCTCGTC7050.020.05597339
GACGAGT7450.019.90915725
CGCGATA3150.019.7130414
GCGCGAT3200.019.40456813
Produced by FastQC (version 0.11.5)