FastQCFastQC Report
Thu 26 May 2016
ERR1356902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356902_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132436
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG4700.032.28606424
TGCGACG5650.026.85743322
CAATGCG5850.025.94044719
GCTCGAT5250.025.63242364
TGGTCGT5700.025.42606751
TCGTCTA5850.025.36397654
CGATAAT5550.024.86568367
CTCGATA5350.024.5077865
ATCGTCA6550.024.25028410
CGTCATT6300.024.08642826
TCGATAA5950.022.03640766
CTGGTCG6500.021.7658350
CATTGCG7050.021.52404229
GCTCGTC6900.021.49213839
AATGCGA7650.021.18832820
ATGCGAC8000.021.12352421
CGCACTA6250.020.98456858
GACGAGT7750.020.9149325
GTCCTAC7400.020.50601230
CACTATC7300.019.84205636
Produced by FastQC (version 0.11.5)