FastQCFastQC Report
Thu 26 May 2016
ERR1356901_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356901_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361347
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG33760.2479896749322546No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG22040.16189847261572546No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC20700.1520552805419926No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA17090.12553742726872721No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA15910.11686954171126099No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT15620.11473929865052776No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG15340.11268251224706119No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT15150.11128683575899459No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA14440.1060714130930615No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCA13810.10144364368526174No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG13770.10114981705619508No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG13650.10026833716899512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAAA1200.043.1541021
TTAAGGT1100.037.6617624
TGGTCGT7400.036.37335251
TCAAGAC2100.034.523283
CTGTGTA3000.034.50551269
GAGTCCT6250.034.2231828
CAATGCG6450.033.69315319
ATCGTCA6300.033.4002810
GCGCTGG8200.032.81387347
GAGATCG751.6727226E-632.19212736
GTCAGCG8400.031.62424343
GTACGCA1101.0604708E-931.35942611
TTCGGTC1001.44973455E-831.0515449
TTTCTAC901.9986328E-730.66142325
GCTCGTC8900.030.6161139
AACCAAG1151.7043931E-929.9992829
GTCTACC9050.029.74174556
ACCTGGG2450.029.5913833
CTGGTCG9350.029.52343650
CGCCAGC9500.029.04553234
Produced by FastQC (version 0.11.5)