FastQCFastQC Report
Thu 26 May 2016
ERR1356901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356901_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361347
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA41270.3031556245395186RNA PCR Primer, Index 46 (96% over 28bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG36520.2682637123378536No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG22710.16682006865259189No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC20900.15352441368732586No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA18200.1336911162253268No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA17340.12737384370039379No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT16020.11767756494119427No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA15050.11055226918632796No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG14960.10989115927092798No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT14910.10952387598459468No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG14600.1072467196093281No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG14560.10695289298026146No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTG14410.10585104312126152No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCA13650.10026833716899512No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG200.00736223651.744825
GCGGGCT200.007367584451.73531726
ACACTTT408.282841E-751.73531716
CACTTTG451.865299E-645.9869517
ATCGTCA7100.044.2496510
AGCGACC551.4733268E-743.89663317
CGACGAG7450.042.12898624
CAATGCG7600.041.75131219
CGTGCTC758.749339E-1041.38825230
TGAACCG602.9133844E-740.245975
GAGTCCT7750.040.0531528
AGTCTAA350.001347108239.43331550
CGGTGTT350.00134978739.41738514
GCGAAGC557.4098134E-637.62568333
CGCAGCG557.4098134E-637.62568314
ACCCTTG1750.037.4836857
TCACCGG852.9776857E-936.51904726
GTCATCC8600.036.4954613
TGGTCGT9050.035.45730651
TCAAGAC1700.034.4965483
Produced by FastQC (version 0.11.5)