FastQCFastQC Report
Thu 26 May 2016
ERR1356899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356899_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420523
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA51840.36493601300366135RNA PCR Primer, Index 33 (96% over 29bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG35850.2523718376963977No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA26400.185847043659272No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC25730.18113047096034346No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA22930.16141942087526917No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA21700.15276063815932583No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA21050.14818485867529071No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG20820.14656573670401676No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19340.13614703880190604No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG18830.13255681182212467No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT17680.12446120196575486No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA16600.11685836836151194No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCACAAGCACGATCGGCTCCTAAT15710.11059307029875616No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTG15220.10714363653386817No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA15130.10651006706684792RNA PCR Primer, Index 33 (96% over 29bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG15130.10651006706684792No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT14630.10299023669451321No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG14250.10031516561153885No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC200.00736797351.7347423
TACCCCG751.6370905E-1145.9912955
ACGTGCT306.3211384E-445.9864416
ATCGTCA7350.043.2196210
GAGTCCT7650.041.92880628
CAATGCG7700.041.20862619
CGACGAG7800.040.6803124
TCAAGAC1200.040.2423823
GCACGAG350.00134801339.42804768
GTCATCC8300.038.2310313
GTTGCGT501.8644822E-434.49104313
GACGTGC400.002600297634.4898315
CGAGAAA400.002600297634.4898323
ATCGCCT807.114795E-834.4898335
GGTCCTA751.6587874E-632.2268078
GGGTAAA1305.456968E-1231.8940181
TGTTGCG553.241555E-431.38974612
GGCGGGC553.25632E-431.36542947
GGTGCAC553.2630496E-431.3543916
CTACACT9650.030.7370533
Produced by FastQC (version 0.11.5)