FastQCFastQC Report
Thu 26 May 2016
ERR1356895_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356895_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2111407
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC45370.21488040913002562No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAG32960.15610443652029193No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGC31220.14786348629136875No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTT30760.14568484427682585No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGCGTTCTATCATTCTAGCCTCG27460.1300554559116267No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAG26430.12517719227036758No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGCCTTATAGAAGGTAAACG26250.12432468017772036No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAAC23890.11314729940745674No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCG22440.10627984088335408No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCATTTCAGATG22250.105379967007782No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATA200.007354922651.7588251
ACGTTGT350.001348358739.42687632
TACCCCG901.2187229E-1038.3507775
CAATGCG6950.034.7483619
GAGTCCT7100.033.52752328
ATCGTCA6950.033.2567810
CGAGTGA958.794814E-932.68745465
TGGTCGT9400.032.67046451
ACCCTTG12650.032.451617
CGACGAG7400.032.16525324
GCTCGAT9450.031.39696764
AACCCTT13100.031.3480076
TCAGCGC10800.030.98698444
GGTTTGC2050.030.29281818
GCGCTGG10600.029.9421147
GTCAGCG10900.029.75313243
ACCGATG703.8486694E-529.57576450
CGTCTTA703.8524064E-529.5715615
GCTCGTC11300.028.98943939
GTCGTCT10750.028.88864353
Produced by FastQC (version 0.11.5)