FastQCFastQC Report
Thu 26 May 2016
ERR1356895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2111407
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC44050.20862865378394596No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAG32790.15529928621056954No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGC32180.15241021745215394No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTT31100.1472951448962706No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGCCTTATAGAAGGTAAACG26960.12768736676538442No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAG26720.12655068397518812No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGCGTTCTATCATTCTAGCCTCG26520.12560344831669118No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAAC23310.11040031599781566No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCG22730.10765333258817462No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCATTTCAGATG21720.10286979251276518No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG552.6047928E-950.170125
CCCGGCG702.1700544E-839.4708028
CCACGGC350.001346636439.4371347
CGACGAG6150.039.2507724
CAATGCG6500.037.667819
CTCGTTG557.420018E-637.6195738
GAGTCCT6550.037.3802628
ATCGTCA6700.036.59885810
AGACTCA852.9813236E-936.51830352
ACCGTAG400.002583228334.5369538
CGGCGCT851.1979319E-732.50536710
ACCCTTG13050.032.2835927
CGCTGCG1001.4433681E-831.06704557
GACTCAC1001.4575562E-831.03614853
TGGTCGT8800.030.97831351
GCGCTGG8950.030.84468347
TGCGACG8000.030.60509122
AACCCTT13900.030.557856
GTCAGCG9150.030.52735743
TTCCTTG10000.030.0016125
Produced by FastQC (version 0.11.5)