FastQCFastQC Report
Thu 26 May 2016
ERR1356893_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356893_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120345
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA8600.032.49044410
TGGTCGT10150.031.96138851
GCTCGAT10450.031.36298264
CAATGCG8950.030.83516319
CTCGATA11550.028.68081765
TCGATAA11350.028.5781666
TGCGACG10000.027.9404622
TCAGCGC11900.027.82938444
GCTCGTC11850.027.64851639
GCGCTGG12550.026.93649947
TCGTCTA12100.026.52536654
GTCGTCT12150.026.41620653
GTCTACC12750.025.71444956
GTCAGCG12550.025.56339543
CGTCATT13000.025.47158226
GTCGTCA13650.024.25979624
CATTGCG14250.023.96848729
CTGGTCG14450.023.88113250
GTCCTAC12000.023.86143930
CGCCAGC14750.023.38437734
Produced by FastQC (version 0.11.5)