FastQCFastQC Report
Thu 26 May 2016
ERR1356893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120345
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG7150.032.3181324
ATCGTCA7800.031.42844210
GCTCGAT11250.030.97638564
CAATGCG7800.030.95363419
TGGTCGT11750.029.95188751
CTCGATA12100.029.08619565
TCGATAA12300.028.61324966
GCTCGTC13050.027.7495739
GTCGTCT12950.027.69752953
TCAGCGC13200.027.69551344
GTCAGCG13250.027.33070843
GCGCTGG13250.027.08191147
GTCTACC12700.026.90015856
TCGTCTA13250.026.82150854
CGTCATT14250.025.89681626
TGCGACG9200.025.49168822
CGCCAGC14950.025.37633734
GTGATCG9150.024.9047368
ACCGTCG8900.024.41338523
GTCGTCA15300.024.34500324
Produced by FastQC (version 0.11.5)