FastQCFastQC Report
Thu 26 May 2016
ERR1356891_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356891_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791055
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG4450.027.90641824
CAATGCG4650.027.44874619
ATCGTCA5350.023.8573210
CTCGATA6400.023.72289865
GCTCGAT6400.023.71759864
TGCGACG5350.023.2118822
TCGATAA6900.022.00384966
CGATAAT7100.021.38402267
TCGTCTA6850.021.16112554
GTACTAG7150.020.7698761
TGGTCGT7150.020.75594351
TCAGCGC7150.020.74492544
GTATCAA53750.020.303931
ATGCGAC7100.019.91989721
TGATCGC4200.019.7122259
GTATAGT4300.019.2758831
TAGGCAT9700.019.2235155
CGCGTTG900.005739101719.1646657
CAACGCA55400.019.135485
GTCGTCT7650.018.94819853
Produced by FastQC (version 0.11.5)