FastQCFastQC Report
Thu 26 May 2016
ERR1356891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791055
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGT7050.033.2849951
CTCGATA7150.031.85062865
TCGTCTA7550.031.53599454
GCTCGAT7250.031.41130864
CTGGTCG8000.029.76375450
GTCGTCT8150.029.19719753
CGATAAT7900.028.8228167
TCAGCGC8150.028.7740544
TCGATAA7950.028.64553366
GCTCGTC8600.027.66943639
CGTCATT9150.026.7600526
GCGCTGG9100.026.1659447
CGCCAGC9950.025.30168534
GTCTACC9700.024.90666456
CGTCTAC9700.024.54605555
CGACGAG4500.024.52376624
CATTGCG10050.024.36362629
AGGTCGT10450.023.4310522
GTCGTCA10700.023.20589824
CAATGCG4800.022.99102819
Produced by FastQC (version 0.11.5)