FastQCFastQC Report
Thu 26 May 2016
ERR1356890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016229
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA63470.6245639516290128No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA58920.5797905786983052No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA53630.5277353824777683RNA PCR Primer, Index 17 (96% over 28bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA50040.4924086992203529No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT33980.3343734532275698No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC29380.28910806520971155No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG23580.23203431510023823No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG23250.22878701552504405No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA22410.22052116206091343No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG21450.21107447238762128No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20100.1977900650345542No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19820.1950347805465107No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC18900.18598170294293906No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA15640.15390231926071782No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA15500.15252467701669603No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA14650.14416042053513528RNA PCR Primer, Index 17 (96% over 28bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG14420.1418971511342424No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA12870.12664468343257276No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG12810.12605426532799202No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC12450.12251175670050746No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA12240.1204452933344748No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT11510.11326187306207557No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG11490.11306506702721533No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG11430.11247464892263456No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC10680.10509442261537508No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG10660.10489761658051483No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA10480.10312636226677255No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA10440.10273275019705205No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGA306.301306E-446.01450746
ATCGTCA3100.043.44686510
GGTCCTA404.9720344E-543.168368
CGACGAG3400.040.56907324
CAATGCG3650.039.6798919
CGATCCT350.001343377239.4546161
GGTTAGC350.001350876139.40995821
GGGGTCC459.9644014E-538.3662076
AGGGGTC451.0036899E-438.319015
GAGTCCT3650.037.7903728
GTCATCC3850.035.83252313
CAACGAG400.002587802234.5227863
AGACTCA400.002599701534.490552
CCGTATC400.002600956734.4871062
GAAGTAG400.002602212134.48371519
GTACTAG5200.031.8734631
ACGACGT652.3108058E-531.86718459
GGATCTC553.2441787E-431.38434857
CCCGCTG450.004599768730.6975049
CTCCCGC450.004603096330.6929657
Produced by FastQC (version 0.11.5)