FastQCFastQC Report
Thu 26 May 2016
ERR1356889_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356889_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625833
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAT4150.026.6036264
TATCGTA658.6610107E-426.537227
CTCGATA4200.026.29014665
CGATAAT4500.026.07106267
TCGATAA4600.024.75417366
ATCGTCA5300.024.73469210
GTCTACC5200.024.55448356
CGACGAG5200.024.54390724
TCGTCTA5200.023.22721554
TGCGACG5500.023.20514922
CAATGCG5400.022.996819
CGTCATT5650.022.58838826
GGTATCA28600.022.5733071
GTACTAG6200.022.2734721
GTATCAA40200.021.8135971
CGCGATA2550.021.6453814
ATGCGAC6550.021.59175121
AGTCCTA5950.021.45409629
AATGCGA6000.021.2720420
ACGAGTC6050.020.52480726
Produced by FastQC (version 0.11.5)