FastQCFastQC Report
Thu 26 May 2016
ERR1356889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625833
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA4650.025.96932865
CGATAAT4800.025.87339667
GCTCGAT5250.023.00140464
TCGATAA5400.022.36247666
TATTACG1403.7822247E-722.1758335
GTATCAA37450.022.0478671
TCGTCTA5600.021.56580754
GCTCGTC6050.020.52323339
TTACTAG3450.019.99445543
GATCGCA4150.019.9653110
CTAACGC2603.6379788E-1219.901393
TAGCGTA3000.019.55660857
TATGCCG23400.019.45614243
CGTCATT6400.019.4008726
CTCGTAT23300.019.39161939
CGTCTAC6800.019.2823755
ACCGTCG3600.019.16135223
GTCTACC6350.019.0204256
TCTCGTA23700.018.91880438
GGTATCA26750.018.8561
Produced by FastQC (version 0.11.5)