FastQCFastQC Report
Thu 26 May 2016
ERR1356887_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356887_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486616
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.027.0028551
CGATAAT1759.094947E-1225.633367
GTATCAA27900.023.510041
TCGATAA2151.4006218E-1020.86431366
CCGTCGA1451.2657683E-519.0335089
GTACTAG2406.039045E-1018.6997621
GTACATG50950.018.2946261
TATCAAC35300.018.2881972
CAACGCA35150.018.1679745
ATCAACG35600.017.9401363
TCGTCTA2701.5097612E-1017.89399754
TCAACGC36400.017.6406884
CTCGATA2551.364242E-917.5914865
TACATGG51800.017.394612
CATTGCG2802.5465852E-1017.25085829
AACGATA1200.00147789317.24853561
AACGCAG37900.017.0255686
ATGCGAC2251.1092561E-716.86523421
GTACCGT1653.8367387E-516.7303586
GTATTAG1704.9346694E-516.2459471
Produced by FastQC (version 0.11.5)