FastQCFastQC Report
Thu 26 May 2016
ERR1356887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486616
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20750.029.1380311
GTATCAA30500.024.9208621
TCGTATG7850.021.96674540
CTCGTAT7800.021.66540739
TATGCCG7950.021.25662643
CGTTAAC1004.344023E-420.6940672
CGTATGC8400.020.52844641
TCTCGTA8600.020.45206338
ATCAACG37250.019.7218633
TATCAAC37550.019.6561492
CAACGCA37300.019.6029595
TCAACGC37650.019.5123334
CGCACTA2002.8616341E-818.98680558
AACGCAG39750.018.3258346
CGTTCGG950.00785151118.15146835
ACGATAG1552.2347731E-517.8175362
ATCTCGT9550.017.69530737
AATCTCG9550.017.69530736
TGCGACG2156.622395E-817.64491722
ATGCGAC2751.9826984E-1017.5574221
Produced by FastQC (version 0.11.5)