FastQCFastQC Report
Thu 26 May 2016
ERR1356883_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356883_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213758
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGT658.6211547E-426.557524
TGCGACG2700.024.2751822
ATCGTCA3100.023.36846710
GTACTAG3850.023.3154321
CGACGAG2950.022.21796224
GTATCAA31450.021.8455641
CAATGCG3350.020.59566119
TCGATAA3050.020.36267766
CTCGATA3050.020.36267765
TTGTACG1409.28631E-619.7284431
CGATAAT3150.019.7162467
ATCAACG34350.019.39823
CGTCACG1451.2629169E-519.03794155
CAACGCA35000.018.9393045
TAGGCAT5500.018.8316965
CTAGGCA6050.018.8316944
ATGCGAC4300.018.45151521
GCTCGAT3400.018.2642664
TCAACGC37300.018.0491454
TGGTCGT3450.018.00327151
Produced by FastQC (version 0.11.5)