FastQCFastQC Report
Thu 26 May 2016
ERR1356883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213758
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG1750.029.56053524
TGCGACG1900.027.22680922
GTACTAG3500.025.6614151
CTAACGG1452.02308E-823.7843427
CGGTCCG750.001997360522.99152828
CAATGCG2550.021.63908419
CAATTCG2900.021.40590515
GTCGACG2501.8189894E-1220.69237536
ATTCGTC2501.8189894E-1220.69237517
TATGCCG16000.020.69237543
CTAGGCA6100.020.3539964
TCGTATG16500.020.06533240
ATCGTCA2603.6379788E-1219.91866910
TCGACGA2603.6379788E-1219.89651537
GTGTACG1409.241279E-619.739551
CTCGTAT16600.019.73670239
CGCGATA1056.0382194E-419.70702614
GTATCAA27750.019.6684151
TCTCGTA16550.019.58794838
CTAACGC2153.1614036E-919.2495143
Produced by FastQC (version 0.11.5)