FastQCFastQC Report
Thu 26 May 2016
ERR1356881_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356881_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598026
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGT8600.036.91456651
GCTCGAT8750.035.0920564
ATCGGTC1154.7293724E-1132.99438526
ATCGTCA7850.032.08437310
CGACACT1950.031.8467214
CAATGCG8150.030.90045419
GCGCTGG10500.030.8833647
TCAGCGC10700.030.62850644
GCTCGTC10250.030.61830739
CGACGAG8150.030.4752524
GTCTACC10450.030.37849856
GTCAGCG10450.030.3708943
CGCCAGC10600.029.93923834
TGCGTAC1800.028.7460160
CTCGATA10850.028.62070765
CGTCATT11300.028.38897526
GTCGTCT11200.028.03701253
GTCATCC9200.027.7504913
TCGATAA11100.027.66525366
CGACTTA500.007736318727.59962332
Produced by FastQC (version 0.11.5)