FastQCFastQC Report
Thu 26 May 2016
ERR1356881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598026
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG350.001338160239.4872321
CGACGAG6850.035.24495724
TGGTCGT8850.035.09184651
CAATGCG6950.033.74532319
GCTCGAT9400.033.03756364
ATCGTCA7450.031.97049310
GCTCGTC10500.031.20497139
CGCCAGC10900.030.37625534
GTCTACC10400.029.86555156
TCAGCGC11100.029.82893644
TTCGGTC1052.3275788E-829.5774149
GCGCTGG11000.029.17408447
CGGCGAT1900.029.04396232
GAGTCCT8350.028.91352728
GTCAGCG11400.028.7414243
CATCGGT1800.028.7414225
CGGTCCG1800.028.7414228
ATCGGTC1800.028.7414226
CGTCATT11450.028.61591326
GTCGTCA11750.027.88529424
Produced by FastQC (version 0.11.5)