FastQCFastQC Report
Thu 26 May 2016
ERR1356878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682688
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA77770.46217718317358897RNA PCR Primer, Index 26 (100% over 29bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG52220.3103367944621938No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC33630.19985879735280693No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA29510.1753741632435722No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA28450.1690747185455652No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG27570.16384499087174806No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA26820.1593878366042903No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT23650.14054893123383538No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG23630.1404300737867032No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG21930.13032719078046553No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT21250.1262860375779705No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCACAAGCACGATCGGCTCCTAAT21060.1251568918302145No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA20670.12283917161113647RNA PCR Primer, Index 26 (100% over 29bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG20580.12230431309904154No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA20560.12218545565190933No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTG19890.11820373117298039No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA19450.11558886733607182No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCT19270.11451915031188195No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT19250.11440029286474973No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTG19160.1138654343526548No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACAT18980.11279571732846494No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG18660.11089399817434961No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCA17930.10655570135402403No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG17900.10637741518332573No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAAC17390.10334655028145442No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTTC16910.10049397155028146No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACACG150.002356094868.9973569
GAGGCTT200.007336545751.79112211
AGTCTAA200.007360737351.74801669
CATGTCG405.0120536E-543.11181636
TACCCCG651.1383236E-842.46375
TTTACAC2250.041.4021072
ATCGTCA7400.041.05962410
CAATGCG7450.040.7416419
CGACGAG7450.040.7392224
CGGTTGG350.00134274339.459910
CTTACGC350.001346281439.4387859
AGTCCTA7800.039.3533529
GAGTCCT8000.038.80063228
GTCATCC7950.038.61427313
TGACGTA557.398381E-637.63492268
CTTAAGG400.002574152334.561341
GTGTAAA400.002574152334.561341
CGTAAGA709.679497E-734.52741210
TTATCAC501.8612282E-434.501762
TGTGTAT905.1913958E-934.501755
Produced by FastQC (version 0.11.5)