FastQCFastQC Report
Thu 26 May 2016
ERR1356877_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356877_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950866
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA558495.8734879572936665No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA70940.7460567524761639No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG252.5682352E-455.19444743
TTATCGA200.007361572251.74479347
AGCCGGA306.3173054E-445.99053634
AAAAAGT4000.037.9741481
TATGTAC4050.037.5053334
TTATGTA4100.037.047953
GCTTATG4150.036.601591
CTTATGT4350.034.918762
TAAGTAC400.002588023434.5219543
ATACCGA501.8571026E-434.5128676
CGACGAG400.002596051734.50015614
CGAATGA501.8611271E-434.50015610
CCGAATG501.8611271E-434.5001569
ATACGTA709.745545E-734.49471325
CCGGACT400.00260007334.4892736
CGGACTG400.00260007334.4892737
AGTACTC5350.034.1975175
AAAAGTA4550.033.3838652
TACGTAG751.6708291E-632.1950726
CTAACGC450.00460793130.686183
Produced by FastQC (version 0.11.5)