FastQCFastQC Report
Thu 26 May 2016
ERR1356877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950866
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA560545.895047251663221No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA434454.56899289700126No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTG150.002349907869.0407912
GGGGCGA200.007365943451.73702242
GCTTATG4400.040.818311
CCGGGGC350.001345530439.441562
CTTATGT4850.036.984312
TTATGTA4900.036.606923
CCCTGCG852.9504008E-936.5510069
TATGTAC5300.035.145834
ATCCGCC601.3397766E-534.509560
CCTAGAC400.002597964434.4949763
CCTGCGT958.742063E-932.7035310
TATGCAC553.2593898E-431.3590683
CGACGCA450.004627967330.6589819
GGTATCG450.004627967330.6589814
TCGACGC450.004627967330.6589818
TATCGAC450.004627967330.6589816
ATCGACG450.004627967330.6589817
AATCCGC854.417616E-628.41958659
GCCGGGG500.00772189227.6090561
CGGGGCC500.007725858627.60614663
Produced by FastQC (version 0.11.5)