FastQCFastQC Report
Thu 26 May 2016
ERR1356875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91518
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG10981.199763980856225No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA9421.029305710352062RNA PCR Primer, Index 46 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA3360.36714089031665903RNA PCR Primer, Index 46 (96% over 28bp)
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGG3130.3420092222295068No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA3030.33108241001770145No Hit
GTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCGTCCCTATACGCGATGGAT1500.1639021831770799No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA1470.16062413951353832No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCT1450.15843877707117726No Hit
GTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGT1370.149697327301733No Hit
GTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGTGCCC1320.14423392119583034No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGGTATTT1270.13877051508992766No Hit
CGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGTGCCCCCGACCTACAACTTGCGCCA1230.13439979020520554No Hit
CCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAG1230.13439979020520554No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA1210.13221442776284448RNA PCR Primer, Index 46 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA1190.1300290653204834RNA PCR Primer, Index 46 (96% over 28bp)
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG1120.12238029677221968No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTC1110.12128761555103913No Hit
GCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCGTCCCTATACGCGATGGATCGAGGTCATGACTG1080.11800957188749753No Hit
GTGTGGTGCCCCCGACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGAGCTGGCGCAAT1040.1136388470027754No Hit
CACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGA1030.11254616578159488No Hit
GCTTCGTGTCGGCACGAAGTGTCTTCCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCC1020.11145348456041435No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGG1010.11036080333923382No Hit
ACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGAGCTGGCGCAATGACGACCTAATTGG990.10817544089687274No Hit
CGTCAGCGCTGGTCGTCTACCAGCTCGATAATTGGCGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTG990.10817544089687274No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC980.10708275967569222RNA PCR Primer, Index 46 (96% over 28bp)
CCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGTGCCCCCGACCTACAACTTGCGCCAATTATCGAGCTG980.10708275967569222No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC940.10271203479097009No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCC940.10271203479097009No Hit
TCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTGGCGCAAGTTGTAGGTCGGGGGCACCAC940.10271203479097009No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGAT920.10052667234860901No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTC150.002344180669.0328112
CTGAGCA150.00234927168.9950919
CCTGAGC150.00234927168.9950918
CTAACTG208.475442E-568.995094
ATTGTAA252.5486803E-455.2262466
CAGCATC252.5555788E-455.19606818
TATTGTA252.5555788E-455.1960685
TCAGCAT252.5555788E-455.19606817
AGCATCG252.5555788E-455.19606819
GCAATAT408.123843E-751.802931
GGTAAAA200.007307900651.802931
TGCGCGT200.00732373351.77460510
CTGCGCG200.00732373351.7746059
CGAGACC200.00732373351.7746058
GAGACCG200.00732373351.7746059
CCGTGAA200.0073395951.74631534
GTCATCC408.1854705E-751.74631513
CCAGCTA200.0073395951.74631523
CTCCTGA200.0073395951.74631516
CCAGTGG200.0073395951.74631556
Produced by FastQC (version 0.11.5)