FastQCFastQC Report
Thu 26 May 2016
ERR1356874_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356874_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356861
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA9960.27910026592987186No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG6650.1863470651037799No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG5820.1630887096096239No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC5590.15664362314738792No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG5340.14963809438408793No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA4720.13226438305110394No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA4650.13030283499737993No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG4650.13030283499737993No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT4520.12665996004046393No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4390.12301708508354794No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG4030.11292912366439593No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC3600.10087961419151995No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCAA200.00735826251.74400714
CAGTTTG200.007366425451.72950435
TCGGTAA200.007366425451.72950439
GTACCCA702.1706E-839.451661
ACCCATG702.1706E-839.451663
CAATGCG901.2187229E-1038.3235219
CATGCTT400.002586504734.5202064
CGTTAAC709.666837E-734.5202031
GAGTCCT1003.783498E-1034.5008428
ATCGTCA905.175025E-934.49600610
CATAAAT958.769348E-932.68042826
CACCACT751.6701233E-632.18724434
GTCATCC1001.4475518E-831.04205113
GTACAGA450.00460524730.684631
AGATCTC450.00461790830.66741832
CTCGGTG450.004624248530.65881736
CACTTAA802.7601127E-630.17977337
GTCCTAC1151.6952981E-930.0007330
TGCGACG1202.6757334E-928.7466722
ATGCAGG500.007716391727.60841452
Produced by FastQC (version 0.11.5)