FastQCFastQC Report
Thu 26 May 2016
ERR1356874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356861
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA15570.43630433137832375TruSeq Adapter, Index 3 (96% over 28bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA10270.2877871215963639No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA7620.2135285167053839TruSeq Adapter, Index 3 (96% over 28bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG6500.1821437478457999No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG5860.1642095942117519No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG5570.15608318084632392No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC5380.1507589789862159No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT4040.11320934481492795No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA3970.11124779676120394No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC3880.10872580640641594No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3860.10816536410535194No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA3830.10732470065375593No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG3820.10704447950322395No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG3630.10172027764311595No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG3580.10031917189045594No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAC150.00235324569.0071565
TGTGTAT309.122723E-657.489845
GACAGAC252.5687975E-455.1825116
ACAGACA252.5687975E-455.1825117
AATAGTC200.00733559651.78439762
AGGTGTC200.00733559651.7843977
AGTCCAA200.007351883751.7553665
ACGTTGA200.00736411751.73360438
GGTCCCT200.00736411751.73360440
TCCTCGA200.00736411751.73360439
AGTCTGC200.00736411751.73360453
CGGTGTT200.00736411751.73360414
CGAATGT200.00736411751.73360443
TGCACAT200.00736411751.73360422
GCACCGG352.2607996E-549.3184746
GCATATA306.2806124E-446.037031
GGAGTCT306.302355E-446.00476551
CAGACAC451.8618284E-645.98542818
ATCGTCA1200.040.27675610
CGACGAG1250.038.6277624
Produced by FastQC (version 0.11.5)