FastQCFastQC Report
Thu 26 May 2016
ERR1356872_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356872_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769579
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG200.00735986351.74968310
CAATGCG8550.031.87699519
ATCGTCA9300.029.30626910
TGCGACG9500.028.32454322
GAGTCCT9650.027.8858428
GTACTAG12600.025.753231
TGGTCGT8400.025.46556951
GACCGTC8400.025.46268822
GTCAGCG8950.025.44118543
ATCGTGG8350.025.201334
ACCGTCG8550.025.01597623
GCTCGAT8900.024.80883664
CCGTCGG8650.024.72677224
CGTGGTT8550.024.20353336
AGACCGT8700.024.18813721
GTCGTCT9150.023.75529553
GTCATCC11550.023.5972613
GTCCTAC11300.023.5080430
CTCGATA9400.023.48988265
CCAATGC11800.023.38968718
Produced by FastQC (version 0.11.5)