FastQCFastQC Report
Thu 26 May 2016
ERR1356872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356872_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769579
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA7800.033.6405210
CGACGAG7800.032.7191524
CAATGCG7700.032.69710519
TGGTCGT8350.032.64665651
GCTCGAT8200.031.56151264
CTCGATA8950.028.91587365
GTCGTCT9450.028.83102853
TATTCGC605.4005894E-428.7706859
GTCAGCG9650.028.23349443
TGCGACG9200.028.11501522
GTCTACC9900.027.5407656
GTCATCC9500.026.86718213
GCGCTGG10400.026.5432947
CTGGTCG10650.026.24421150
ATCGTGG8550.026.21875634
CGTGGTT8650.025.91565336
GCTCGTC10600.025.70313539
GACCGTC8750.025.6194722
TCGTCTA11200.025.57378454
TCGATAA10150.025.49724866
Produced by FastQC (version 0.11.5)