FastQCFastQC Report
Thu 26 May 2016
ERR1356871_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356871_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112281
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32000.029.0253031
GTATCAA49850.025.3507251
CGTACGA2005.2750693E-1122.42581760
TGCGACG2950.022.22272522
CAACGCA56850.021.985285
ATCAACG56900.021.9063173
CGATAAT2850.021.78665467
TCAACGC57600.021.7000394
AACGCAG59200.021.3854086
TATCAAC59800.021.0171892
CGCACTA2850.019.36912558
TAGCGTA2505.0931703E-1119.32253557
CGTAACG900.00573241419.16872623
ACGCAGA64700.019.1404917
GTACATG58350.018.8174481
CGCAGAG65300.018.8061438
TACATGG57550.018.6590482
GAGTACT37600.018.44043412
TCGTCTA3650.017.96423754
ATCGTCA3650.017.95700610
Produced by FastQC (version 0.11.5)