FastQCFastQC Report
Thu 26 May 2016
ERR1356871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112281
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30450.025.20141
GTATCAA46500.024.9029521
CGATAAT2900.021.41359567
ATCAACG54850.020.127913
CAACGCA55650.019.714575
CGTACGA2655.456968E-1219.53366960
TATCAAC58250.019.4268882
TCAACGC57000.019.2476444
AACGCAG57400.019.1704446
CGACTAT2255.5515557E-918.39721736
TACGTCG950.00784340618.15514828
TATGCCG5250.017.74017343
CGTCATT3700.017.71353726
TCGTCTA4150.017.46263154
GTACATG57150.017.4194811
TCGTATG5750.017.39736740
GTCGACG1402.0803754E-417.25351750
TCTCGTA6250.017.10941138
CTCGTAT6100.016.96464539
TACATGG56800.016.9465482
Produced by FastQC (version 0.11.5)