FastQCFastQC Report
Thu 26 May 2016
ERR1356870_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356870_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001019
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG6150.031.97504224
ATCGTCA6300.031.2121710
CAATGCG6550.030.02086619
GTCCTAC7650.024.80099930
GTATCAA86250.024.6999441
GGTATCA54200.024.653671
GTACTAG9700.023.8491541
TGGTCGT6500.023.3575251
CGTATCG750.001995481322.9961438
ACGAGTC8450.022.86235426
ACCGTCG5200.022.55729323
GTCAGCG6800.022.320943
GTCGTCT6500.022.29581553
TGCGACG8750.022.07960722
AGTCCTA8600.022.06135629
ATGCGAC9400.021.65387221
GAGTACT53100.021.5691612
GTCCGTT800.00290131521.56103912
CGTGGTT5650.021.36809736
GCTCGTC7450.021.29843939
Produced by FastQC (version 0.11.5)