FastQCFastQC Report
Thu 26 May 2016
ERR1356870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001019
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG4350.040.440524
CAATGCG4800.036.649219
ATCGTCA5900.030.40093210
GTCTACC7150.028.48084856
GTACTAG8200.028.2435911
TGGTCGT7450.027.7923951
GTCGTCT7400.027.50146953
CCCCGTA1555.2750693E-1126.70454225
TGCGACG6850.026.688322
ACCGTCG6250.026.49090623
GTCAGCG7850.026.36435543
CGATAAT7850.025.93207767
ACGAGTC6850.025.68119226
GCTCGTC8200.025.65969839
CTCGATA7950.025.60652765
TCGTCTA8150.024.98626754
GCGCTGG8450.024.91174147
TCAGCGC8550.024.2058744
TCGTGGT6550.023.69773335
GTCCTAC7450.023.6129130
Produced by FastQC (version 0.11.5)