FastQCFastQC Report
Thu 26 May 2016
ERR1356868_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356868_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142035
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1150.044.9959718
GTTCGAC1250.041.3926716
CGACGAG2100.041.06775324
CGCTCCG1400.039.41986534
CAATGCG2350.036.69884519
TTCGACC1450.035.68489517
ATCGTCA2500.034.4984210
AAGTTCG1500.034.49540314
TCGAGTA501.8628694E-434.495425
CCGCCAG1550.033.38118738
TGCGACG2600.033.1701122
GCGCTCC1750.031.53727333
GCGTAGC553.259561E-431.35945567
ATGCGAC3250.030.78185821
ACGAGTC2850.030.25912526
GGGCCGT1750.029.5674944
GGGGCCG1700.028.40797865
GCCCGTT1103.667992E-828.22350962
CGTCATC3350.027.804712
GGCGTAC500.00773992927.59632162
Produced by FastQC (version 0.11.5)