FastQCFastQC Report
Thu 26 May 2016
ERR1356868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142035
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG1750.043.3656524
ATCGTCA2050.037.01945510
TGCGACG2100.036.13804222
CTCGATA2200.034.50295665
TCGACCG905.1950337E-934.49691418
CCGTCGC400.002598025634.49540327
CAATGCG2250.033.73031219
GCTCGAT2350.032.3006464
GTTCGAC1052.3330358E-829.56878316
GCAAGCG605.426407E-428.7461742
TGGTCGT2950.028.07142851
AAATGCG500.00772505727.60719957
ACGAGTC2900.027.35842326
GTACTAG3950.027.1174581
GTCGTCT3050.026.01292653
GTCAGCG2950.025.72538443
ATGCGAC3400.025.36426521
AAGTTCG1251.2655983E-724.83777814
TCGTCTA3200.024.80334554
GCTCGTC3200.024.7935739
Produced by FastQC (version 0.11.5)