FastQCFastQC Report
Thu 26 May 2016
ERR1356867_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356867_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541639
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.10333158411275274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG4550.037.1551424
CAATGCG5050.034.842819
ATCGTCA5000.032.42481610
TGGTCGT6600.030.84798851
GAGTCCT5400.030.66375528
GTCAGCG6600.030.31431843
GCTCGAT6500.030.25097364
GCTCGTC7150.028.46213339
TATTCTC7250.028.098725
GTCTACC7250.028.0795756
GTCGTCT7400.027.97939353
CGCCAGC7550.027.866134
TCTCTGC7350.027.689458
TACGGGA500.007700175527.6260622
GAACCGT500.00773930827.5973830
GGACGTA500.00774175927.5955896
GGGTAAA1653.6379788E-1227.2074851
ACGCAAG1404.6929927E-1027.102818
GTCATCC6000.027.0206813
TCGATAA7500.026.67746766
Produced by FastQC (version 0.11.5)