FastQCFastQC Report
Thu 26 May 2016
ERR1356867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356867_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541639
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAT404.9996994E-543.12956262
CCGAATT404.9996994E-543.12956263
CGTGCCT350.001348764839.42379440
CGACGAG5850.038.91835824
ATCGTCA5950.038.26426710
CAATGCG6250.037.53266519
TGGTCGT5500.036.38802751
GTCAGCG5800.035.68532643
GCTCGAT6000.034.5014164
CGAGTGA400.002597326634.49805565
GAGTCCT6800.033.48123628
GTCATCC6750.033.2192713
GCGCTGG6600.031.89180647
TATTCGC652.3239589E-531.842299
GTCGTCT6200.031.71389653
TGCCGAA553.2549002E-431.36797161
CAGATCG450.004617851730.6738960
TCGCTGG703.8550454E-529.56784412
CTACACT7450.029.17095833
GTCTACC7100.029.16179856
Produced by FastQC (version 0.11.5)