FastQCFastQC Report
Thu 26 May 2016
ERR1356865_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356865_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578087
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG37480.23750274858103512No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG32560.20632576024008814No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT26340.16691094977653323No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA24790.1570889310918853No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT24400.15461758445510293No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC23070.14618965874505016No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC22850.144795565770455No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG19390.12287028535182154No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT19370.12274354962685834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19310.12236334245196874No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT17130.10854914843098004No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA16430.10411339805726806No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAT200.007364792751.74065816
GGTGTCG350.001347486739.4314522
CAATGCG6500.037.15538419
CGACGAG6750.036.2915424
GTCTCCG601.3433588E-534.49814611
TCGCTGG601.3436504E-534.4970512
GAGTCCT7150.034.2547328
ATCGTCA7150.033.7744110
TTCGGTC1800.032.58984449
CGGTCCA1800.032.58984451
TCAAGAC2150.032.111663
TGGTCGT8150.031.75481251
GTCAGCG9150.030.91538843
GTCATCC7700.030.91196313
TGATTCG450.00462442330.6650166
TAAGCGT450.00462442330.6650166
GGTATCA52300.029.4386671
GTCGTCT9550.028.18364153
GTCCTAC8500.028.00171330
CATGGGT3850.027.7953514
Produced by FastQC (version 0.11.5)