FastQCFastQC Report
Thu 26 May 2016
ERR1356865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578087
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG40360.2557526929757358No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT26810.1698892393131684No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT26680.16906545710090762No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC24030.15227297354328373No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG20730.13136157892435588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17620.11165417369257842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA7200.035.9322710
CGACGAG7350.035.19895624
CAATGCG7100.034.98192619
TGGTCGT9800.034.8591251
GTACACG501.8415743E-434.5639721
GTACGTG400.002598481734.49497635
TTCGGTC1650.033.46133849
CGGTCCA1650.033.46133851
GTCAGCG10650.033.3613443
GAGTCCT7800.033.16824728
GTCTACC10600.032.2292656
GTCGTCT10900.031.96323853
TCAGCGC11650.031.08989344
GCTCGAT10350.030.6709564
TGAACCG901.9957588E-730.6670635
CGGCGAT450.00462650130.66220532
TCGCTGG1359.094947E-1230.66220512
GCTCGTC11950.030.59805739
GCGCTGG11450.030.43849447
CCGTGTT802.765646E-630.1831049