FastQCFastQC Report
Thu 26 May 2016
ERR1356858_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356858_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931757
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTT200.007355156451.75823655
ACCATCG306.314827E-445.99659710
CAATGCG7900.039.7375719
CGACGAG8150.038.52161824
ATCGTCA8750.036.6658610
TTCGGTC1051.6370905E-1136.1476749
CACCGTA400.002596392834.50102247
GAGTCCT9100.034.49655528
TCTTACG501.8629788E-434.49655528
GTCATCC9500.034.13343413
CGTGGTT7250.030.92474436
GTCCTAC10100.030.0556330
GTACTAG12200.029.7150521
AGTCCTA10300.029.47202329
TGGTCGT9300.029.31111751
TCGGTCC1301.9645086E-1029.19695550
ATCGTGG7650.028.85538134
CGGTCCA1202.6830094E-928.75457451
ACCGTCG7700.028.67543223
TCATCCA12100.028.5095514
Produced by FastQC (version 0.11.5)