FastQCFastQC Report
Thu 26 May 2016
ERR1356858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931757
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG309.108109E-657.5215455
CGGCGAT252.5700993E-455.1892332
TCACGAG200.007365466651.73990653
CGTGATG306.317791E-445.9922215
GCACCGC306.317791E-445.9922221
CATCGGT405.009058E-543.1165925
CAATGCG7150.039.07730519
ATCGGTC451.00297E-438.32585526
CGACGAG7350.038.0129924
ATCGTCA7850.036.03305410
ACCGTGT1151.8189894E-1235.9948438
TGAACCG1550.035.613335
GAGTCCT8050.035.13600528
TGGTCGT10650.033.37523751
GTCAGCG10750.033.3702343
GAACCGT1305.456968E-1231.841596
CGCCAGC11500.031.79379734
ACTCCCG553.2605807E-431.3591426
GCTCGTC11900.030.72509839
TGGCGCC450.00461499930.67815655
Produced by FastQC (version 0.11.5)