FastQCFastQC Report
Thu 26 May 2016
ERR1356857_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356857_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647858
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG8600.032.49440824
CAATGCG8600.032.09263619
GCTCGAT11200.029.26138164
CGATAAT11500.028.1991367
ATCGTCA10300.028.13292510
TGGTCGT12050.027.77564851
CTGGTCG13250.025.26011850
CTCGATA13150.025.18553265
CGATATA1651.1641532E-1025.0887162
GTCAGCG13650.025.02025843
TCGTCTA13900.024.57489854
AGTCCTA11750.024.36853829
GCTCGTC14550.023.94409439
GTCGTCT14250.023.72961653
TCGATAA13950.023.49389666
GTCTACC14950.023.31049556
TGCGACG11900.023.19343822
GTCCTAC12200.023.18693230
ATTCGCT1653.1523086E-922.99755110
CGGGATA8450.022.86708550
Produced by FastQC (version 0.11.5)