FastQCFastQC Report
Thu 26 May 2016
ERR1356857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647858
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAA553.2609914E-431.3590016
TGGTCGT11850.031.15906351
GCTCGAT11850.030.28201364
CGATAAT11800.030.11507267
GTCAGCG12800.029.10507243
CTCGATA12700.028.79645765
CAATGCG9500.028.687419
GCTCGTC13400.028.3167139
CGACGAG9650.027.8818924
CTGGTCG13300.027.76202250
TCGTCTA13500.027.35279854
CGATATA1306.3027983E-926.5435662
TCAGCGC14750.026.19273844
GTCTACC14050.026.03642356
GTCGTCT14350.025.96131953
TAAATCG1208.539064E-825.87117624
TCGATAA14000.025.6291466
GCGCTGG14800.024.94830347
ATCGTCA11150.024.74970410
CGTCATT16000.024.57761826
Produced by FastQC (version 0.11.5)